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Analitical techniques in combinatorial chemistry - Swarth M.E.

Swarth M.E. Analitical techniques in combinatorial chemistry - Marcel Dekker, 2000. - 311 p.
ISBN 0-8247-1939-5
Download (direct link): analyticaltechniquesincombinatorialchemistry2000.pdf
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VI. CONCLUSION
LC characterization of combinatorial libraries is a vital tool in drug discovery, and, in one format or another, will remain so for a long time. High throughput methods, open access environments, mass directed auto-purification, and total system integration have lead to increased utilization of LC in ways that a few short years ago were unimaginable. This trend can only continue, as researchers strive for higher throughput, and wider applicability in solving challenges in drug discovery.
ACKNOWLEDGMENTS
The author would like to acknowledge the contributions of Chris Chumsae, Andrew Brailsford, Jeffrey Holyoke, and Beverly Kenney of Waters Corporation who collaborated in various ways in the data generated for use in this chapter.
Liquid Chromatography
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REFERENCES
1. IM Mutton. Chromatogr 708(47):1-8, 1998.
2. JN Kyranos and JC Hogan, Jr. Anal Chem 70(11):389A-395A, 1998.
3. WK Goetzinger and JN Kyranos. Am Lab April:27-37, 1998.
4. R Cole, KA Laws, DL Hiller, JP Kiplinger, and RS Ware. Am Lab July:15-20, 1998.
5. ME Swartz, M Balogh, and B Kenney. Poster presented at the Eastern Analytical Symposium, Somerset NJ, November 1998, and Waters Corporation (Milford, MA) Literature Code M29.
6. JP Kiplinger, RO Cole, S Robinson, EJ Roskamp, RS Ware, HJ O’Connell, A Brailsford, and J Batt. Structure controlled automated purification of parallel synthesis products in drug discovery. Rapid Commun Mass Spectrom 12:658-664,
1998.
7. L Zeng, L Burton, K Yung, B Shushan, and DB Kassel. Automated analytical/ preparative HPLC-MS system for the rapid characterization and purification of compound libraries. J Chromatogr A 794:3-13, 1998.
8. HN Weller, MG Young, SJ Michalczyk, GH Reitnauer, RS Cooley, PC Rahn, DJ Loyd, D Fiore, and SJ Fischman. Mol Div 3:1-24, 1997.
9. LR Snyder, JJ Kirkland and JL Glajch. Practical HPLC Method Development. Second Edition. New York: John Wiley and Sons, Inc., 1997.
10. JL Li and JMorawski. LC/GC Magazine 16(5):468-476, 1998, and Waters Corporation (Milford, MA) Literature Code Number T158.
11. C Chumsae and A Brailsford. Poster presented at ASMS, Houston, TX. June
1999.
12. Brochure No. BR25/DAM, Micromass Corporation, Manchester UK, April 1999.
13. A Stolyhwho, H Colin, and G Guichon. J Chromatogr 265:1-18, 1983.
14. A Stolyhwho, H Colin, M Martin, and G Guichon. J Chromatogr 288:253-275, 1984.
15. TH Mourey and LE Oppenheimer. Anal Chem 56:2427-2434, 1984.
16. M Lafosse, C Elfakar, L Morin-Allory, and M Dreux. J High Res Chromatogr 15:312-318, 1992.
17. CE Kibbey. Mol Div 1:247-258, 1995.
18. A Brailsford and C Chumsae. Poster presented at ASMS, Houston TX. June 1999.
19. EW Taylor, MG Qian, and GD Dollinger. Anal Chem 70:3339-3347, 1998.
20. R Bizanek, JD Manes, and EM Fujinari. Peptide Res 8:40-44, 1996.
21. WL Fitch, AK Szardenings, and EM Fujinari. Terahedron Lett 38:1689-1692, 1997.
22. ME Swartz and BF Kenney. Poster presented at the Eastern Analytical Symposium, Somerset, NJ, November 1998.
23. SA Cohen, BF Kenney, J Holyoke, TA Dourdeville, and D Della Rovere. LC/ GC, 17(4S):S9-S16, 1999.
136
Swartz
24. ME Swartz. Unpublished results, 1999.
25. PA Keifer. DDT, 2(11):468-478, 1997.
26. FS Pullen, AG Swanson, MJ Newman, and DS Richards. Rapid Comm Mass Spectr 9:1003-1006, 1995.
27. SH Smallcombe, SL Patt, and PA Keeifer. J Mag Res, Series A 117:295-303, 1995.
28. H Barjat, GA Morris, MJ Newman, and AG Swanson. J Mag Res, Series A 119: 115-119, 1996.
6
Capillary Electrophoresis in Combinatorial Library Analysis
Ira S. Krull
Northeastern University Boston, Massachusetts
Christina A. Gendreau
Waters Corporation Milford, Massachusetts
Hong Jian Dai
Shuster Laboratories Quincy, Massachusetts
I. INTRODUCTION AND OVERVIEW: WHY HPCE FOR COMBINATORIAL MAPPING ANALYSIS?
Although capillary electrophoresis (CE),1 also known as high-performance CE or HPCE, has been known and described in the literature for almost two decades, its use for combinatorial mapping is much more recent (1-14). There does not appear to be a previous review, other than that in Analytical Chemistry (an American Chemical Society journal), that describes the general applicability and applications of CE for these purposes (15). There are relatively few actual publications in the refereed literature that have utilized various CE modes to perform analysis of combinatorial maps. At the same time, there are
1 All acronyms and abbreviations are defined in the glossary preceding the references.
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many more CE papers that have used affinity recognition to identify an active antigen or antibody or receptor molecule, but not as part of a larger library of similarly structured compounds. Most papers in the literature deal with the synthesis and characterization of libraries in terms of chemical structures, not biological activity (15-17). Because sections of this book are devoted to analytical approaches to screen compounds in combinatorial libraries for activity, it represents the first major effort to describe how analytical chemistry can be applied to identify individual, active members of a chemical library. In this chapter, the focus is on describing how CE approaches (methods, instrumentation, protocols, techniques) can be applied to a combinatorial library and isolate, as well as characterize, only those active (lead) compounds in a given library. This is quite different from characterizing all of the members of a given library in terms of their individual structures or saying that a given structure is present in a particular library.
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