# liquid chromatography column - Scott R.P.W.

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peak, asymmetry. 41 capacity, 67

effect of capacity factor. 71

equation for, 70

detector sensitivity, effect of. 73 optimized column, 202 equation for, 203 definition. 9 height, definition. 11 maximum, definition. 11 shape, effect of isotherm shape. 44 width, definition. 11 measurement of. 50 equation for, 46 performance criteria, for column design, 176

peptides, diffusion coefficients, 270 phase, mobile, 4 stationary. 4 plastic unions, 7 plate, effective plates, 63 equation for, 64

relationship to equilibrium plates, 65 height. 97

height, reduced, 126 theory. 12,15 theoretical, definition. 17 volume, definition, 18 point, of inflexion, of the elution curve. 48

position, 49 of injection, definition. 9 point, dead, 10 Poiseuille's equation, 132 Poisson equation, form of elution curve, equation for. 21 axes of. 51 polar forces, 5 interactions, 5 pore volume, definition of, and expression for, 29 power, resolving, of column, 60 equation for. 62 precision as an alternative to resolution. 74

preparative columns, 237 design, 238

optimum particle diameter, 239 equation for. 240 optimum column iengtn, 241 equation for. 242 program for the design of, open tube columns. 232 packed columns. 208

program for the design of (cont.)

preparative columns. 252 protocol for column design. 175 Purnell equation. 62, 185^

quality of packing and reduced plate height. 127 quartz columns, availability. 7

random walk model, equation for, 98 radial dispersion. 99 equation for. 100 radial equilibrium, column, 100 radius, column, effect of extra column dispersion. 167

packed, equation for. 169. 170 optimum. 196 equation for. 198 optimum, open tubular columns, 218 equation. 219 packed column, 196 equation. 198 preparative column, 244 equation. 246 rate theory, 12. 93 validation data. 139 equations, 135 ratio, capacity, 25

calculated from, exclusion volume. 151

permeating volume, 150 equation for. 25 explicit equation for, 32 optimum, 204 phase. 25 equation for, 25 separation. 26 equation for, 26 reduced, chromatogram. 177 plate height. 126 velocity. 126 report from computer design, of an open tube column. 233 of a packed column. 210 of a preparative column. 256 resistance to mass transfer, packed column, mobile phase. 105 equation. 106 relation to diffusivity, 147 particle size. 148

stationary phase. 106 equation. 106 resolving power, ol column. Û) equation for, 62 Giddings definition. 65 relationship to effective plates. 66 terms of minimum separation ratio, 66

terms of effective plates, 66 resolution, column, see resolving power retention, factors controlling. 4 time, adjusted, definition. 11 definition, 11 volume, adjusted, definition. 11 equation for, 24 volume, definition. 11

derivation of equation for. 22 effect of molecular size of solute. 36 equation for. 23 explicit equation for. 31 peak composition. 76

sample volume, effect on dispersion, 153 maximum. 53 equation for. 54

optimized open tube column. 226 equation for, 228 optimized packed, column, 204 equation for, 204 overload, 259 selectivity, factors controlling. 4 sensitivity, mass. 172 separation ratio. 26 equation lor. 26 plates required, 60 equation for, 62 serpentine tubes, low dispersion, 161 SFC.6

silica gel. importance to liquid

chromatography. 2 specifications, analytical, for optimum column. 182. 183 solvent, minimum consumption, for packed columns, 201 equation for. 201 for preparative columns. 248 equation for. 248 physical properties of. 271 static phase, definition. 29 stationary phase. 4

279

summation of variances. 94 symbols, general, chromatographic, 12 list of. 265

thermostat, column, 9 theoretical plate, definition, 17 theory, plate, 12. 15 theory, rate, 12, 93 thermodynamic dead volume. 31 time, analysis, approximate for a packed column, 117 minimum for open tube column, 222 equation for. 223 minimum, for packed column. 194 equation for. 195 minimum for preparative column, 241

equation for. 242 particle diameter, 121 dead, definition, 10 retention, definition, 11 adjusted retention, definition. 11 Tswett, inventor of chromatography, I tubes, connecting, effect on dispersion. 154

length versus variance, 157 low dispersion. 158 coiled tubes. 159 serpentine tubes, 161 pressure drop across. 156 low aspect ratio, elution profile of, 165

unavailable stationary phase, definition, 30

unions, plastic, 7 vacancy, chromatography. 55 applications. 59 equation for elution curve, 59 validation data for rate equations, 139 valve, sample, elution profile. 152 variance, summation of. 94 per unit length, 97 equation for. 97 Van Deemter equation. 109 H versus u curves, 138 validation of, 135 variables, elective, in column design. 181 velocity, mobile phase optimum, open tube column. 217. 131 optimum, packed column. 113

velocity, mobile phase, approximate value, 114 reduced,126 volume, finite charge, curve for. 39 column, total, expression for. 28 dead, definition. 10 discussion of, 27 kinetic, 31 thermodynamic, 31 effective cell, heat of adsorption detector. 79 interstitial, definition of. and expression tor. 29 effect of ion volume of marker, 34 moving. 29 static, 29 mobile phase, expression for, 29 plate, definition. 18 pore, definition of, and expression for,

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