# liquid chromatography column - Scott R.P.W.

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Appendix 1 The Diffusion Coefficients of some Low Molecular

Weight Substances ............................................................................................268

Appendix 2 The Diffusion Coefficients of some Peptides ..................................270

Appendix 3 The Physical Properties of some Solvents

Employed as Mobile Phases in LC ....................................................271

Index

273

Index

adjusted retention time, definition. 11 retention volume, definition, 11 adsorption, detector, heat, equation for output, 83

equation for on column detection, 87 theory of, 77 isotherms. 43 alumina, coated stationary phase, 2 limitations as an LC stationary phase, 2 analysis time, approximate for packed column, 117

minimum, optimized open tube columns, 222 equation for. 223 minimum, optimized, packed columns, 194

equation for, 195 minimum, optimized, preparative columns, 242 equation for. 242 particle diameter, 121 analytical specifications for optimum column. 183 asymmetry, peak. 41 available stationary phase, definition, 30 axis of a chromatogram, 96

base line, definition, 10

capacity peak, 67

and capacity factor, 71 equation for, 70 optimized column, 20 equation for, 203 ratio. 25

calculated from, exclusion volume, 151

permeating volume. 150 effect on dispersion, 149 equation for, 25 explicit equation for, 32 cell detector, dispersion effect, 164 design for low dispersion, 166 volume, effective, heat of adsorption detector. 79 charge, finite, elution curve of, 39 chromatogram, axes of, 96 reduced. 177 chromatorgaphy, nomenclature, 9 vacancy, 55 applications. 59 equation of elution curve, 59 column, capillary, 6 characteristics of, 1 dead volume, 10,27 design, open tubular volumns, 215 optimized open tube columns, 231 program for. 232 optimized packed columns, 2(16 program for, 208 optimized preparative columns, 250 program for, 252 packed.185

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optimum particle diameter. 239 diameter versus particle diameter. 171 dispersion, extra, 153 packed.102

longitudinal diffusion. 103 equation for. 104

dispersion (ñîò.)

multipath, 102 equation for. 103 resistance to mass transfer, mobile phase. 106 stationary phase, 106 environment, 8 efficiency, 45 equation for, 46, 47 frits, contribution to dispersion. 164 flow rate, definition, 10

optimum for open tube. 224 optimum for packed, 199 optimum for preparative, 247 heat capacity, 8

length, approximate for packed column, 117

optimum for open tube column, 221 equation for, 222 optimum for packed column. 192 equation for, 192 optimum for preparative column, 241

equation for, 241 particle diameter for maximum efficiency, 120 particle limits, 8 material of construction, 6 open tubular, optimum, design, 215 flow rate, 224 equation for, 225 radius. 218 equation, 219 velocity, 217 minimum HETP, 217 limitations, 6, 233 overload, 259 packed,6

optimum, specifications for, in column design. 182 physical nature of, 6

properties of Zorbax column, 37 purpose of, 3 quartz, availability, 7 radial equilibrium, 100 radius, dependance on extra column dispersion. 167 equation for, 169, 170 optimum, open tubular, 218 equation for. 219 packed,196 equation for, 198

preparative, 244 equation for, 246 resolving power, 60 equation for. 62 theories, 12 thermostat. 8

volume, total, expression for, 28 wall thickness, limitations. 8 preparative, 248 weight, limits, 8 computer program for the design of open tube columns. 232

report for a packed column design, 233 computer program for the design of packed columns, 208 report for a packed column design, 210 computer program for the design of preparative columns, 252 report for a packed column design, 256 connecting tubes, dispersion, cause. 154 equation for. 155 length versus variance, 157 low dispersion, 158 coiled tubes, 159 H/u curves, 161 serpentine tubes, 161 H/u curves, 162. 163 pressure drop across, 156 constraints, instrument, in column design, 179 criteria for column performance, 176

d'Arcy’s law. 116 dead, point, definition, 10 time definition, 10 volume, definition. 10 discussion of, 27 kinetic, 31 thermodynamic, 32 design, equations for open tube columns, 231

program for. 232 equations for packed columns, 206 program for. 208 equations for preparative columns, 250 program for. 252 open tubular columns. 215 packed columns. 185 preparative columns, 237 protocol for columns. 175 detector, cell, design for low dispersion, 166

dispersion, effect on, 164 heat of adsorption, theory, 77 equation of output, 83 equation for on column detection,

87

differential equation for the elution curve,18 diffusion coefficients, list of. 268 peptides. 270 diffusivity relation to, longitudinal diffusion, 143 minimum HETP. 145 resistance to mass transfer. 147 dipole, induced, 5 interactions. 5 dispersion, detector cell dispersion, effect on, 164

effect of sample volume, 153 extra column, 6,95, 153 packed column, 102 radial. 99 equation for, 100 dispersive forces, 5 dispersive interactions. 5 distribution, 4 coefficient, 16 factors controlling. 4

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