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Introductionth to Cell and Tissue Culture - Jennie P.

Jennie P. Introductionth to Cell and Tissue Culture - Plenum Press, 2002.
ISBN 0-306-45859-4
Download (direct link): introductiontocellandtissue—Āulture2002.pdf
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^jlycine
óL"CHistidme"CHCrCH20 10.81 óHydroxyproline óL'CIsoleucine 26
óL'CLeucine 26
F12 DMEM F12"CDME
8.9 4.45
211 84 147.5
15 7.5
13.3 6.65
35.1 17.56 63 31.29
14.7 7.35
146 584 365
7.5 30 18.75
21 42 31.48
4 105 54.47
13.1 105 59.05
F10
9
211
15
13
25
14.7
146
7.5
23
2.6
13
20
0.3
5
3330-
Page 45
Media
Med 199 RPMI 1640 Way mouth L15 CMRL 1 Grace's Insect Media
200-
25 225 25 225-
70 200 75 500 70 700-
50 250 350-
30 20 60 30 350-
0.1 100 120 260
26 65 20 26 22-
75 20 150 75 600-
100 300 350 300 600-
50 10 50 200 50 650-
21.88 15 164 250 20 2500-
10 20 10
40 50 25 250 20 50-
60 50 50 125 60 75-
óL' CLysine hydrochloride 36.47 29 36.5 146 91.25
óDL'CMethionine
óL'CMethionine 7.5 4.5 4.5 30 17.24
óDL'CPhenylalanine
óL'CPhenylalanine 16.5 5 5 66 35.48
óL'CProline 11.5 34.5 17.25
óDL"C Serine
óL'CSerine 10.5 10.5 42 26.25
óDL'CThreonine
óL'CThreonine 24 3.6 11.9 95 53.45
óL'CTryptophan 4 0.6 2.04 16 9.02
óL"CTyrosme"C2Na"C2H20 26 2.62 7.81 104 55.79
óL'CValme 23.5 3.5 11.7 94 52.85
Vitamins
óAscorbic acid
óAlpha" Ctocopherol
óBiotm 1 0.024 0.0073 0.0035
^^alciferol (vitamin D2)
óD'CCalcium pantothenate 1 0.7 0.5 4 2.24
Choline chloride 1 0.7 14 4 8.98
70 40 240 75 70 625-
15 15 50 75 15 50-
25 15 50 125 25 150-
40 20 50 40 350-
550-
25 30 200 25
300
30 20 75 30 175-
10 5 40 20 10 100-
58 29 58 300 58 50-
25 20 65 100 25 100-
0.05 17.5 50
0.01
0.01 0.2 .02 0.01 0.01
0.01 0.25 1 1 0.01 0.02
0.5 3 250 1 0.5 0.2
(icontinued)
Page 46
Table 4.1 (Continued)
Components BME F10
óFolic acid 1 1.3
óL'CInositol 2 0.5
óMenadione (vitamin K3)
óNiacin
óNiacinamide 1 0.6
óPara'Caminobenzoic acid
óPyridoxal hydrochloride 1
óPyridoxine HCl 0.2
óRiboflavin 0.1 0.4
Riboflavin 5'"Cphosphate, Na
óThiamine hydrochloride 1 1
Thiamine
monophosphate
óVitamin A (acetate)
óVitamin B12 1.4
F12
1.3
18
0.036
0.06
0.037
0.3
1.4
DMEM
4
7.2
4
0.4
F12"CDME
2.65
12.6
2.02
2
0.219
2.17
0.68
Media Med 199 RPMI 1640
0.01
0.05
0.01
0.025
0.025
0.05
0.025
0.025
0.01
0.01
0.14
1
35
1
1
1
0.2
0.005
Waymouth
.4
1
1
1
10
L15
1
2
0.1
CMRL 1 0.01 0.05
0.025
0.025
0.05
0.025
0.025
0.01
0.01
ďThis table compares the composition and concentrations of components of several media. Note the qualitative and quantitative differences in the media.
4
1
1
1
4
1
1
Grace's Insect Media 0.02 0.02
0.02
0.02
0.02
0.02
0.02
Page 47
Table 4.2 Commonly Used Mediaa Media
Basal media Eagle (BME)
Minimal essential media (MEM)
Dulbecco's Modified Eagle's media (DMEM)
Ham's F10 media
Ham's F12 nutrient mixture (F12)
F12"CDME: (1:1) mixture
William's media E RPMI 1630
RPMI 1640
Leibovitz L"C15 medium Waymouth's MB 752/1 Fischer's media McCoy's 5A media MCDB 131 Media 199
Medium NCTCC109 Media NCTCC135 Neurobasal medium BGJb medium Glasgow minimal media CMRL media
Applicable to
Growing cells with serum
Growing cells with dialysed serum
Many virus transfected cells, growth with serum, high-density growth
CHO cells, low density, low serum protein
Serum growth, many cells, serum free
Rat liver epithelial cells
Mouse leukemia cells, cells in suspension
Human leukemic (and other) cells Buffered for air, human tumors L cells
Murine leukemia cells Human lymphocytes Human endothelial cells Chick embryo fibroblasts Hybridomas
Serum, serum-free growth CNS neurons Fetal rat long bones BHK"C21
Reference Eagle (1965)
Eagle (1959)
Dulbecco and Freeman (1959)
Ham (1963)
Ham (1965)
Mather and Sato (1979); Bottenstein et al. (1979)
Williams and Gunn (1974)
Moore and Kitamura (1968) Leibovitz (1963)
Waymouth (1959)
Fischer and Sartorelli (1964)
McCoy et al. (1959)
Knedler and Ham (1987)
Morgan et al. (1950)
Evans et al. (1956)
Evans et al. (1964)
Brewer et al. (1994)
Biggers et al. (1961)
Macpherson and Stoker (1962) Parker et al. (1957)
L-cells, monkey kidney cells
aThis table lists some of the commonly used media, the use for which they were originally derived, and the original reference. It is apparent that many of these media are used much more widely than originally intended. This is one reason that the time spent testing several media for optimal growth of a different cell type is time well spent.
More recently, vendors are supplying "special-use media" to grow a stated cell under special conditions. These sometimes contain undisclosed hormones, growth factors, or undefined protein components. Therefore, these cannot be considered "defined," although they may work well for some applications. Other such media are supplied with a defined supplement mix that must be added before use. A list of some commonly used media and the uses for which they were originally developed can be found in Table 4.2. Some special-use media and their suppliers are listed in Table 4.3. Tables 4.2 and 4.3 may be used as a rough guide to what media may be useful for what cells; however, many of these media are now used to grow a wide variety of cells. The optimization of the medium for specific cells and uses is discussed below.
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