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Chromatographic scince series - Cazes J.

Cazes J. Chromatographic scince series - Marcel Dekker, 1996. - 1098 p.
ISBN 0-8247-9454-0
Download (direct link): сhromatography1996.pdf
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Synergic in vitro effects of some mixtures can be determined by Hill reaction inhibition. This method is useful only when the components have quite different herbicidal activities and one of them is not a Hill reaction inhibitor. With the help of paper chromatography, a 40% synergic effect of an atrazine-defenuron (3:2) mixture was detected (166).
Semiquantitative analysis of prometryne, simazine, and atrazine was carried out in soil samples by their Hill reaction inhibitory effect (167) (Table 12). A chronometric method was developed for the quantitative determination of these herbicides in food samples with recoveries between 70% and 100%
(168). This method was extended for the determination of residues of atrazine, lenacyl, and chloridazon in commercial sugar samples with recoveries, 90,90, and 60%, respectively (169) (Table 12).
Triazine-type herbicides were detected in the lower nanogram range by this photosynthesis inhibition reaction with chloroplasts obtained from parts of oats, spinach, beans, and watercress (170);
values in five different solvent systems are given in Table 12. This useful, sensitive method for residue analysis was patented (171) for the quantitative determination of photosynthesis-inhibiting herbicides.
2. Fung/tests
Fungitests are bioassays that can be applied in the TLC detection of fungicides. These are sensitive methods not only for the detection of pesticides but also in the research for fungicidal activity.
TABLE 12 Hill Reaction
R, x 100
Compound Stationary phase Mobile phase (!) (2) (3) (4)
Bentazone Silica gel with- Chloroform: hexane DNOC out polyacryl- (3:7)
Dinoseb amide binder
Propanil
Terbacil
Linuron
Chloroxuron
Diuron
Atrazine
Metribuzin
Simazine
Cyanazine
Swep
Solan
Prometryne Silufol Acetone-water
Atrazine (Kavalier) (99.5:0.5)
Simazine
Prometryne Silica gel (1) Benzene-EtOH 42 44 49 74
Simazine (9:1) 28 17 24 61
Terbutryne (2) Ѳ- 41 5 48 75
(5) (6)
Detection
Limit of detection
(ng) Sample
Ref.
Spinach or bean leaf chloroplast, 2,6-dichloro-phenol indophenol (Hill r.)
38 51
13 33
6 47
I01
103
10'
1.00
1.00
0.06
0.10
0.20
0.40
1.00
1.00
1.00
1.00
1.00
500
350
220
75
75
75
Potatoes 164
Carrots
Com
Soil
167
168
808 Fodor-Csorba
Terbuthylazine (9: 1) 36 17
Metribuzine (3) CHCl,-EtOAc- 42 48
Chlobromuron acetone 38 58
Linuron (17:2: 1) 38 55
Mitoxuron (4) Light petrolem 13 7
Chloroxuron acetone 21 21
(7:3)
(5) --
hexane-EtOAc
(15:2:3)
(6) CHCl,-EtOAc-
acetone
(13:5:3)
Atrazine CHCl,:MeOH (9:1)
Lenacil
Chloridazone (1) (2)
Atrazine Kieselgel 60 (1) CHCI racetone 42 13
Aziprotryne F,54 (95:5) 70 39
Desmetryne (2) Hexane-acetone 31 09
Methoprotryne (90:5) 23 09
Propazine (3) Hexane-ethyl- 50 21
Simazine octane (90 : 10) 31 07
Terbutryne (4) CHCl,:ethyi- 60 21
Terbuthylazine ocatane (90: 10) 51 21
(5) Toluene: acetone
(85: 15)
47
60
66
62
16
25
(3)
06
27
02
01
10
03
10
10
75 36 53 50
61 51 59 25
64 59 57 25
64 56 55 25
23 11 21 75
32 17 30 75
0.003 Sugar 169
[mg/kg]
(4) (5)
35 56 5-50 Carrots 170
79 85 Potatoes
26 43 No cleanup
12 38
45 63
23 47
52 65
45 63
Pesticides 609
810
Fodor-Csorba
Culvularia lunata spores were grown on TLC plates, where inhibition zones developed. Semi-quantitative determination of the fungicides was carried out by comparing the areas with known standards. The detection limit of this method was about 10-30 ng (110)
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